DNA sequences - SNP detection
This project originated from fruitful meetings of the INRA biodiversity working group (DGAP) concerning the obtention and management of genetic variability data. genetic variability is shown by polymorphous markers. Markers data combined to phenotypic data give the opportunity to discover genotype/phenotype associations which in turn can be used in genetic improvement programs and in biodiversity management. SNPs (Single Nucleotide Polymorphisms) are the more numerous genetic markers with a relatively homogeneous distribution and hence are very good markers. High-throughput sequencing and ESTs programs provides most of the data within which it is possible to identify candidate SNPs. To date, there is no publicly available automated system for SNP detection.
In order to provide such a system, we initialised the development of a SNP detection pipe-line. This system permits the detection via two different strategies :
SNP detection in EST databases
SNP detection in sequences produced by direct DNA sequencing.